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王小元 (2014/03/05)

编辑: 发布日期:2017-03-28 来源:食品科学与技术国家重点实验室  发布日期:2013/10/2

研究方向负责人:

王小元教授

教育部长江学者特聘教授

清华大学硕士

英国伦敦大学博士

美国杜克大学博士后

曾先后在英国和美国从事研究工作十年;2007年至今全职在江南大学工作,任食品科学与技术国家重点实验室食品安全方向负责人,国家自然科学基金委员会专家评审组成员,国家食品药品监督管理局保健食品安全专家委员会委员。任Springer出版社Subcellular Biochemistry系列丛书的国际顾问编委,任国际学术期刊Biotechnology and Applied Biochemistry和Journal of Food Processing and Preservation编委。

研究组主要成员:

副教授:

李烨;史锋;胡晓清;李颜颜[f1]

Tel/Fax:+86-510-85329329

Email:xiaoyuanwang@hotmail.com

实验室网页:

http://biotech.jiangnan.edu.cn/detail.asp?n_id=147

http://wanglab.jiangnan.edu.cn/cnversion.php

研究领域:

1. 食源性病原微生物的致病机制

食源性病原微生物是食品安全的首要隐患。这些微生物通过各种方式提高其在食品加工和储藏过程中的生存和繁殖能力,危害人体健康。本方向重点研究病原微生物细胞膜和细胞壁内关键分子的结构及合成途径,以及这些分子在菌膜形成和群体效应中的功能,并在此基础上提出检测和控制食源性病原微生物的新策略。

2. 微生物代谢工程优化

代谢工程正在取代经典的随机突变而成为工业菌种改良的主要策略。本研究方向主要利用微生物的基因组信息,通过代谢工程技术来优化工业微生物生产菌种。一方面通过敲除或高效表达目标产物合成和调控中涉及到的关键基因来提高产量;另一方面通过优化细胞膜和细胞壁的结构来增强产物的定向通透或消除其反馈抑制。目前正在对异亮氨酸、缬氨酸、甲硫氨酸、苏氨酸和亮氨酸等生产菌种进行代谢工程改造优化。

3. 食源性生物毒素的清除和干预

传统发酵食品主要采用天然发酵或混菌发酵方式,体系中某些微生物会产生对人体有害的毒素。本方向一方面研究上述毒素的微生物合成途径,通过基因工程构建不产生毒素的安全食用微生物;另一方面通过优化发酵体系中益生菌的表层分子组成和结构,提高其吸附或转化生物毒素的能力。

主要论著

Books, Chapters and Reviews:

1. Wang, X., Chen J and Quinn PJ. (2012) Reprogramming microbial metabolic pathways. Subcell Biochem 64. Springer. (book)

2. Wang, X. and Quinn, P. J. (Eds.) (2010) Endotoxins: structure, function and recognition. Subcell Biochem. 53. Springer. (book)

3. Quinn, P. J. and Wang, X. (Eds.) (2008) Lipids in health and disease. Subcell Biochem, Vol. 49. Springer. (book)

4. Dong, X., Quinn, P.J., and Wang, X. (2012) Microbial metabolic engineering for L-Threonine production. Subcell Biochem 64, 283-302. (chapter)

5. Hu, X., Quinn, P.J., Wang, Z., Han, G. and Wang, X. (2012) Genetic modification and bioprocess optimization for S-Adenosyl-L-methionine Biosynthesis. Subcell Biochem 64, 327-342. (chapter)

6. Wang, X. and Quinn, P. J. (2010) Endotoxins: Lipopolysaccharides of gram-negative bacteria. Subcell Biochem 53, 2-36. (chapter)

7. Wang, X., Zhang, C., Shi, F. and Hu, X. (2010) Purification and characterization of lipopoolysaccharides. Subcell Biochem 53, 37-72. (chapter)

8. Dong, X., Quinn, P.J., and Wang, X. (2011) Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for the production of L-threonine. Biotechnol Adv. 29, 11-23. (review)

9. Wang, X. and Quinn, P. J. (2010) Lipopolysaccharide: Biosynthetic pathway and structure modification. Prog Lipid Res. 49, 97-107. (review)

10. Shi, F., Li, Y., Li, Y., and Wang, X. (2009) Molecular properties, functions, and potential applications of NAD kinases. Acta biochimica et biophysica Sinica 41:352-61. (review)

11. Wang, X. and Quinn, P.J. (2000) The location and function of vitamin E in membranes. Mol Membr Biol, 17, 143-156. (review)

12. Wang, X. and Quinn, P.J. (1999) Vitamin E and its function in membranes. Prog Lipid Res, 38, 309-336. (review)

13. Shi, F., Li, Y., Li, Y., and Wang, X. (2009) Molecular properties, functions, and potential applications of NAD kinases. Acta biochimica et biophysica Sinica 41,352-61. (review)

Research Papers:

1. Shi, F., Li, K., Huan, X., and Wang, X. (2013) Expression of NAD(H) kinase and glucose-6-Phosphate dehydrogenase improve NADPH supply and L-isoleucine biosynthesis in Corynebacterium glutamicum ssp. lactofermentum. Appl. Biochem. Biotechnol. Jul 19

2. Shi, F., Zhan, W., Li, Y., and Wang X. (2013) Temperature influences β-carotene production in recombinant Saccharomyces cerevisiae expressing carotenogenic genes from Phaffia rhodozyma. World J. Microbiol. Biotechnol. Jul 17.

3. Hu, J., Tan, Y., Li, Y., Hu, X., Xu, D., and Wang, X.( 2013) Construction and application of an efficient multiple-gene-deletion system in Corynebacterium glutamicum. Plasmid. doi:pii: S0147-619X(13)00077-2. 10.1016/j.plasmid.2013.07.001.

4. Han, Y., Li, Y., Chen, J., Tan, Y., Guan, F. and Wang, X. (2013) Construction of monophosphoryl lipid A producing Escherichia coli mutants and comparison of immuno-stimulatory activities of their lipopolysaccharides. Mar. Drugs. 11(2), 363-76.

5. Cai, L., Li, Y., Tao, G., Guo. W., Zhang, C. and Wang, X. (2013) Identification of three genes encoding for the late acyltransferases of lipid A in Cronobacter sakazakii. Mar. Drugs. 11(2), 377-86.

6. Yin, L., Shi, F., Hu, X., Chen, C., and Wang, X. (2013) Increasing l-isoleucine production in Corynebacterium glutamicum by overexpressing global regulator Lrp and two-component export system BrnFE. J Appl Microbiol. 114(5),1369-77.

7. Li, Y., Powell, D.A., Shaffer, S.A., Rasko, D.A., Pelletier, M.R., Leszyk, J.D., Scott, A.J., Masoudi, A., Goodlett, D.R., Wang, X., Raetz, C.R., and Ernst, R.K. (2012) LPS remodeling is an evolved survival strategy for bacteria. Proc. Natl. Acad. Sci. U S A. 109(22), 8716-21.

8. Yin, L., Hu, X., Xu, D., Ning, J., Chen, J., and Wang, X. (2012) Co-expression of feedback-resistant threonine dehydratase and acetohydroxy acid synthase increase L-isoleucine production in Corynebacterium glutamicum. Metab. Eng. 14(5),542-50.

9. Shi, F., Huan, X., Wang, X., and Ning, J. (2012) Overexpression of NAD kinases improves the l-isoleucine biosynthesis in Corynebacterium glutamicum ssp. lactofermentum. Enzyme Microb. Technol. 51(2),73-80.

10. Wang, L., Hu, X., Tao, G. and Wang, X. (2012) Outer membrane defect and stronger biofilm formation caused by inactivation of a gene encoding for heptosyltransferase I in Cronobacter sakazakii ATCC BAA-894. J Appl Microbio. 112(5),985-97.

11. Yang, Y., Shi, F., Tao, G., and Wang, X. (2012) Purification and structure analysis of mycolic acids in Corynebacterium glutamicum. J. Microbiol. 50(2),235-40.

12. Kanistanon, D., Powell, D.A., Hajjar, A.M., Pelletier, M.R., Cohen, I.E., Way, S.S., Skerrett, S.J., Wang, X., Raetz, C.R., Ernst, R.K. (2012) Role of Francisella lipid A phosphate modification in virulence and long-term protective immune responses. Infect. Immun. 80(3),943-51.

13. Ren, K., Li, Y., Shi, F. and Wang, X. (2012) Separation of lipopolysaccharides containing different fatty acid chains using hydrophobic interaction chromatography. Anal. Methods. 4, 838-43.

14. Tan, Y., Xu, D., Li, Y., and Wang, X. (2012) Construction of a novel sacB-based system for marker-free gene deletion in Corynebacterium glutamicum. Plasmid 67, 44–52.

15. Kong, Q., Six, D. A., Roland, K. L., Liu, Q., Gu, L., Reynolds, C. M., Wang, X., Raetz, C. R. H. and Curtiss III, R. (2011) Salmonella synthesizing 1-Monophosphorylated lipopolysaccharide exhibits low endotoxic activity while retaining its immunogenicity. J. Immunol.187(1), 412-23.

16. Li, L., Wang, X. and Ernst, R.K. (2011) A rapid one-step method for the characterization of membrane lipid remodeling in Francisella using matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry. Rapid Commun. Mass Spectrom. 25, 2641–8

17. Guan, Z., Wang, X., and Raetz, C.R.H. (2011) Identification of a chloroform-soluble membrane miniprotein in Escherichia coli and its homolog in Salmonella typhimurium, Analytical Biochemistry 409(2),284-9.

18. Pan, L., Hu, X., and Wang, X. (2011) Assessment of antibiotic resistance of lactic acid bacteria in Chinese fermented foods. Food Control 22,1316-21.

19. Xu, D., Tan, Y., Li, Y., and Wang, X. (2011) Construction of a novel promoter-probe vector and its application for screening strong promoter for Brevibacterium flavum metabolic engineering. World J. Microbiol. Biotechnol. 27, 961-8.

20. Chen, J., Tao, G., and Wang, X. (2011) Construction of an Escherichia coli mutant producing monophosphoryl lipid A. Biotechnol. Lett. 33, 1013

21. Dong, X., Quinn, P.J., and Wang, X. (2011) Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for the production of L-threonine. Biotechnol. Adv. 29, 11-23.

22. Ma, P., Hu, X., Chen, J., and Wang, X. (2011) The effect of the structure of lipopolysaccharide on the permeability of Escherichia coli cell membranes. Microbiology China. 38(8), 1307−15

23. Wang, X. and Quinn, P. J. (2010) Lipopolysaccharide: biosynthetic pathway and structure modification. Prog. Lipid Res. 49, 97-107.

24. Xu, D., Tan, Y., Shi, F., and Wang, X. (2010) An improved shuttle vector constructed for metabolic engineering research in Corynebacterium glutamicum. Plasmid. 64, 85–91

25. Zhang, C., Li, Y., Tao, G., Li, Y. and Wang, X. (2010) Structural characterization of lipid A in food borne pathogen Cronobacter sakazakii. Eur. J. Mass Spectrom. 16(4), 531-8

26. Wang, X., Guan, Z., Li, Y., and Raetz, C.R.H. (2010) Application of electrospray Ionization mass spectrometry to characterize glycerophospholipids in Francisella tularensis subsp. Novicida. Int. J. Mass Spectrom. 293, 45-50.

27. Li, Y., Wu, S., Wang, L., Li, Y., Shi, F. and Wang, X. (2010) Differentiation of bacteria using fatty acid profiles from gas chromatography–tandem mass spectrometry. J. Sci. Food Agric. 90(8),1380-3.

28. Xu, D., Tan, Y., Huan, X., Hu, X. and Wang, X. (2010) Construction of a novel shuttle vector for use in Brevibacterium flavum, an industrial amino acid producer. J. Microbiol. Meth. 80, 86-92.

29. Peng, Z., Fang, J., Li, J., Liu, L., Du, G., Chen, J., Wang, X., Ning, J., and Cai, L. (2009) Combined dissolved oxygen and pH control strategy to improve the fermentative production of L-isoleucine by Brevibacterium lactofermentum. Bioprocess Biosyst. Eng. 33,339-45.

30. Raetz, C.R., Guan, Z., Ingram, B.O., Six, D.A., Song, F., Wang, X., and Zhao, J. (2009) Discovery of new biosynthetic pathways: the lipid A story. J. Lipid Res. 50, S103-8

31. Wang, X., Ribeiro, A. A., Guan, Z. and Raetz, C. R. H. (2009) Identification of undecaprenyl phosphate-β-D-Galactosamine in Francisella novicida and its function in lipid A modification. Biochemistry. 48, 1162–72.

32. Rao, Z., Ma, Z., Shen, W., Fang, H., Zhuge, J., Wang, X. (2008) Engineered Saccharomyces cerevisiae that produces 1,3-propanediol from D-glucose. J. Appl. Microbiol. 105(6),1768-76.

33. Li, Y., Li, Y., Shi, F. and Wang, X. (2008) Rapid detection of Francisella tularensis by multiplex PCR. J. Biotechnol. 136S, S765.

34. Wang, X., Mansourian, A. R., Quinn, P. J. (2008) The effect of dolichol on the structure and phase behaviour of phospholipid model membranes. Mol. Membr. Biol. 25(6-7), 547-56

35. Wang, X., Ribeiro, A. A., Guan, Z., Abraham, S. & Raetz, C. R. H. (2007) Attenuated Virulence of a Francisella Mutant Lacking the Lipid A 4′-Phosphatase. Proc. Natl. Acad. Sci. U S A. 104, 4136-41.

36. Wang, X., Ribeiro, A. A., Guan, Z., McGrath, S., Cotter, R. & Raetz, C. R. H. (2006) Structure and biosynthesis of free lipid A molecules that replace lipopolysaccharide in Francisella tularensis subsp. novicida. Biochemistry 45, 14427-40.

37. Wang, X., McGrath, S.C., Cotter, R.J., Raetz, C.R. (2006) Expression cloning and periplasmic orientation of the Francisella novicida lipid A 4′-Phosphatase LpxF. J. Biol. Chem. 281, 9321-30.

38. Wang, X., Quinn, P.J. (2006) The structure and phase behaviour of alpha-tocopherol-rich domains in 1-palmitoyl-2-oleoyl-phosphatidylethanolamine. Biochimie. 88, 1883-8.

39. Wang, X, Karbarz, MJ, McGrath, SC, Cotter, RJ, Raetz, CR. (2004) MsbA transporter dependent lipid A 1-dephosphorylation on the periplasmic surface of the inner membrane:Topography of Francisella novicida LpxE expressed in Escherichia coli. J. Biol. Chem. 279, 49470-8.

40. Tran, A.X., Karbarz, M.J., Wang, X., Raetz, C.R., McGrath, S.C., Cotter, R.J., Trent, M.S. (2004) Periplasmic cleavage and modification of the 1-phosphate group of Helicobacter pylori lipid A. J. Biol. Chem. 279, 55780-91.

41. Wang, X., Bogdanov, M. and Dowhan, W. (2002) Topology of polytopic membrane protein subdomains is dictated by membrane phospholipid composition. EMBO J.21, 5673-81.

42. Wang, X. and Quinn, P.J. (2002) The phase behaviour of 1-palmitoyl-2-oleoyl-phosphatidylcholine in the presence of -tocopherol. Biochim. Biophys. Acta 1567,6-12.

43. Wang, X. and Quinn, P.J. (2002) Cubic phases are induced by cholesterol in dispersions of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine. Biochim. Biophys. Acta 1564, 66-72.

44. Wang, X. and Quinn, P.J. (2002) Phase separations of alpha-tocopherol in aqueous dispersions of distearoylphosphatidylethanolamine. Chem. Phys. Lipids 114, 1-9.

45. Wang, X. and Quinn, P.J. (2000) The location and function of vitamin E in membranes. Mol. Membr. Biol. 17, 143-56.

46. Wang, X., Semmler, K., Richter, W. and Quinn, P.J. (2000) Ripple phases induced by alpha-tocopherol in saturated diacylphosphatidylcholines. Arch. Biochem. Biophys. 377, 304-14.

47. Wang, X. and Quinn, P.J. (2000) The distribution of alpha-tocopherol in mixed aqueous dispersions of phosphatidylcholine and phosphatidylethanolamine. Biochim. Biophys. Acta 1509, 361-72.

48. Wang, X. and Quinn, P.J. (2000) Preferential interaction of alpha-tocopherol with phosphatidylcholines in mixed aqueous dispersions of phosphatidylcholine and phosphatidylethanolamine. Eur. J. Biochem. 267, 6362-8.

49. Wang, X. and Quinn, P.J. (1999) The effect of alpha-tocopherol on the thermotropic phase behaviour of dipalmitoylphosphatidylethanolamine-A synchrotron X-ray diffraction study. Eur. J. Biochem. 264(1), 1-8.

50. Wang, X., Takahashi, H., Hatta, I. And Quinn, P.J. (1999) An X-ray diffraction study of the effect of alpha-tocopherol on bilayers of dimyristoylphosphatidylethanolamine. Biochim. Biophys. Acta 1418, 335-43.

51. Wang, X. and Quinn, P.J. (1999) Inverted hexagonal and cubic phases induced by alpha-tocopherol in fully hydrated dispersions of dilauroylphosphatidylethanolamine. Biophys. Chem. 80,93-101.

52. Wang, X., Liu, Y. and Sun, X. (1995) Excess molar enthalpies of n-alkanol plus n-hexane mixtures under high-pressure. Thermochim. Acta 253, 51-8.

上一篇:刘龙 (2014/03/05)

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