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吴敬 (2014/03/05)

编辑: 发布日期:2017-03-28 来源:食品科学与技术国家重点实验室  发布日期:2013/10/2

M.E. & B.E. in Fermentation Engineering, Wuxi Institute of Light Industry

Ph.D. in Microbial and Biochemical Pharmaceutics, China Pharmaceutical University

Research fellow in Molecular Biology, Department of Medicinal Chemistry, College of Pharmacy, University of Michigan

Research Investigator in structure biology, Life Sciences Institute, University of Michigan

Tel0510-85327802

Fax0510-85326653

Email: jingwu@jiangnan.edu.cn

研究领域和兴趣:

运用分子生物学、基因工程、发酵工程和酶工程开发新型发酵产品及功能性食品,具体研究内容包括:新型酶制剂的挖掘及编码基因鉴定、酶催化与转化、基因工程高效表达系统的构建、蛋白质分子设计与定向改造、发酵过程优化与控制以及氨基酸代谢工程。

Research area and interest:

Our Laboratory is committed to explore new types of fermentation products and functional food using technology of Molecular Biology, Genetic Engineering, Fermentation Engineering, and Enzymatic Engineering. The specific research area including: enzyme discovery and gene identification, enzyme catalysis and conversion, efficient expression system development, molecular design and reconstruction of proteins, fermentation process optimization and control, and metabolic engineering of amino acids.

主要论著:

1 Sheng Chen, Lingqia Su, Jing Wu*, Jian Chen. Cutinase: characteristics, preparation, and application. Biotechnology Advances. 2013. DOI 10.1016/j. biotechadv.2013.09.005

2 Xuguo Duan, Jian Chen, Jing Wu*. Optimization of pullulanase production in E. coli by regulation of process conditions and supplement with natural osmolytes. Bioresource Technology. 2013.146: 379–385

3 Lei Wang, Dan Wu, Jian Chen, Jing Wu*. Enhanced production of γ-cyclodextrin by optimization of reaction of γ-cyclodextrin glycosyltransferase as well as synchronous use of isoamylase. Food Chemistry. 2013.141(3):3072-3076

4 Lingqia Su, Chenhua Xu, Ronald W Woodard, Jian Chen, Jing Wu*. A novel strategy for enhancing extracellular secretion of recombinant proteins in Escherichia coli. Applied Microbiology and Biotechnology. 2013.97(15):6705-6713

5 Lingqia Su, Ronald W Woodard, Jian Chen, Jing Wu*. Extracellular location of Thermobifida fusca cutinase expressed in Escherichia coli BL21(DE3) without mediation of a signal peptide. Applied and Environmental Microbiology. 2013.79(14):4192-4198

6 Xuguo Duan, Jian Chen, Jing Wu*. Improving the thermostability and catalytic efficiency of Bacillus deramificans pullulanase by site-directed mutagenesis. Applied and Environmental Microbiology. 2013.79(13):4072-4077

7 Yao Zhang, Lei Wang, Jian Chen, Jing Wu*. Enhanced activity towards PET by site-directed mutagenesis of Thermobifida fusca cutinase-CBM fusion protein. Carbohydrate Polymers. 2013.97(1):124–129

8 Yufei Wu, Shuguang Yuan, Sheng Chen, Dan Wu, Jian Chen, Jing Wu*. Enhancing the production of galacto-oligosaccharides by mutagenesis of Sulfolobus solfataricus β-galactosidase. Food Chemistry. 2013.138(2-3):1588- 1595

9 Xuguo Duan, Sheng Chen, Jian Chen, Jing Wu*. Enhancing the cyclodextrin production by synchronous utilization of isoamylase and α-CGTase. Applied Microbiology and Biotechnology. 2013.97(8):3467-3474

10 Jing Cheng, Dan Wu, Sheng Chen, Jian Chen, Jing Wu*. High-Level Extracellular production of α-cyclodextrin glycosyltransferase with recombinant Escherichia coli BL21(DE3). Journal of Agricultural and Food Chemistry. 2011.59:3797–3802

11 Yao Zhang, Sheng Chen, Meng Xu, Artur Cavoco-Paulo, Jing Wu*, Jian Chen*. Characterization of Thermobifida fusca cutinase–carbohydrate-binding module fusion proteins and their potential application in bioscouring. Applied and Environmental Microbiology. 2010.76(20):6870-6876

12 Dongli Chen, Xing Tong, Shangwei Chen, Sheng Chen, Dan Wu, Shuguang Fang, Jing Wu*, Jian Chen*. Heterologous expression and biochemical characterization of alpha-glucosidase from Aspergillus niger by Pichia pastroris. Journal of Agricultural and Food Chemistry. 2010.58(8):4819-4824

13 Zhaofeng Li, Zhengbiao Gu, Miao Wang, Guocheng Du, Jing Wu*, Jian Chen*. Delayed supplementation of glycine enhances extracellular secretion of the recombinant α-cyclodextrin glycosyltransferase in Escherichia coli. Applied Microbiology and Biotechnology. 2010.85(3):553-561

14 Zhaofeng Li, Bin Li, Zhiguo Liu, Miao Wang, Zhengbiao Gu, Guocheng Du, Jing Wu*, Jian Chen*. Calcium leads to further increase in the glycine-enhanced extracellular secretion of the recombinant enzyme in Escherichia coli. Journal of Agricultural and Food Chemistry. 2009.57: 6231-6237

15 Zhaofeng Li, Jiayu Zhang, Miao Wang, Zhenbiao Gu, Guocheng Du, Jianke Li, Jing Wu*, Jian Chen*. Mutations at Subsite -3 in Cyclodextrin Glycosyltransferase from Paenibacillus macerans enhancing α-cyclodextrin specificity. Applied Microbiology and Biotechnology. 2009.83(3):483-490

16 Zhaofeng Li, Jiayu Zhang, Qi Sun, Miao Wang, Zhengbiao Gu, Guocheng Du, Jing Wu*, Jian Chen*. Mutations of lysine 47 in cyclodextrin glycosyltransferase from Paenibacillus macerans enhance β-cyclodextrin specificity. Journal of Agricultural and Food Chemistry. 2009.57:8386–8391

17 Jing Wu, Weishu Bu, Kelly Sheppard, Makoto Kitabatake, Suk-Tae Kwon, Dieter Söll, Janet Smith*. Insights into tRNA-dependent amidotransferase evolution and catalysis from the structure of the Aquifex aeolicus enzyme. Journal of Molecular Biology. 2009.391(4):703-716

18 Sheng Chen, Xing Tong, Ronald W. Woodard, Guocheng Du, Jing Wu*, Jian Chen*. Identification and Characterization of Bacterial Cutinase. Journal of Biological Chemistry. 2008.283(38):25854-25862

19 Dongxu Zhang, Miao Wang, Guocheng Du, Qingxin Zhao, Jing Wu*, Jian Chen*. Surfactant protein of the Streptomyces Subtilisin inhibitor family inhibits transglutaminase activation in Streptomyces hygroscopicus. Journal of Agricultural and Food Chemistry. 2008.56:3403-3408

20 Zhaofeng Li, Miao Wang, Feng Wang, Zhengbiao Gu, Guocheng Du, Jing Wu*, Jian Chen*. γ-Cyclodextrin: a review on enzymatic production and applications. Applied Microbiology and Biotechnology. 2007.77(2):245-255

21 Jing Wu, Ronald W. Woodard*. New insights into the evolutionary links relating the 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase subfamilies. Journal of Biological Chemistry. 2006.281(7):4042-4048

22 Jing Wu, Galina Ya Sheflyan and Ronald W. Woodard*. Bacillus subtilis 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase revisited: resolution of two longstanding enigmas. Biochemical Journal. 2005.390(2):583-590

23 Jing Wu, Mayur A. Patel, Appavu K. Sundaram and Ronald W. Woodard*. Functional and biochemical characterization of a recombinant Arabidopsis thaliana3-deoxy-D-manno-octulosonic 8-phosphate synthase. Biochemical Journal. 2004.381(1):185-193

24 Jing Wu, David L. Howe, Ronald W. Woodard*. Thermotoga maritima 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase: the ancestral eubacterial DAHP synthase? Journal of Biological Chemistry. 2003.278(30):27525-27531

25 Jing Wu, Ronald W. Woodard*. Escherichia coli YrbI is 3-deoxy-D-manno- octulosonate 8-phosphate phosphatase. Journal of Biological Chemistry. 2003.278(20):18117-18123

上一篇:刘龙 (2014/03/05)

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