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副教授

刘松

编辑: 发布日期:2018-04-25 来源:
职称 副教授 研究方向 酶分子高效表达与改造

姓  名:刘 松

性  别:男

出生日期:1981.08

职  称:副教授,硕士生导师

电  话:0510-85918307

E-mail: liusong@jiangnan.edu.cn

 

教育经历:

1999.09-2003.07郑州粮食学院,本科

2005.09-2007.07江南大学食品学院,硕士

2007.09-2011.12江南大学生物工程学院,博士

工作经历

2003.08-2005.07罗牛山股份有限公司,技术员

2012.02-2016.09江南大学生物工程学院,讲师

2012.09-2012.10日本大阪大学,访问学者

2016.07-至今江南大学生物工程学院,副教授

研究方向:

酶分子高效表达与改造

科研项目:

[1] 国家自然科学基金面上项目“基于蛋白质折叠调控的链霉菌谷氨酰胺转胺酶高效分泌表达研究”(31771913)主持

[2] 国家自然科学基金青年基金项目“融合自组装双亲短肽提高Pseudomonas aeruginosa脂肪氧合酶热稳定性机制的研究”(31401638)主持

[3] 江苏省自然科学基金青年基金项目“调控Streptomyces hygroscopicus谷氨酰胺转胺酶折叠速率强化异源分泌的研究”(BK20130132)主持

[4] 863项目子课题“有机酸生物合成途径构建与优化技术”(2015AA021003)主持

[5] 江苏省重点研发计划社会发展项目“耐热脂肪氧合酶高效制备关键技术”(BE2016629)参与

研究生招生专业:

发酵工程

代表性学术成果(论文、专利等):

学术论文

在Applied Microbiology and Biotechnology、Microbial Cell Factories、FEMS Microbiology Letters等生物工程类国际期刊上,以第一或责任作者(含共同责任作者)发表SCI(E)论文18篇。

[1] Feng Y,Liu S*, Jiao Y, Gao H, Wang M, Du G, Chen J*.2017. Enhanced extracellular production of L-asparaginase fromBacillus subtilis168 byB-subtilisWB600 through a combined strategy.Applied Microbiology and Biotechnology101:1509-1520.

[2] Lu X, Wang G, Feng Y,Liu S*, Zhou X, Du G, Chen J.2016. The N-Terminal alpha-Helix Domain ofPseudomonas aeruginosaLipoxygenase Is Required for Its Soluble Expression inEscherichia colibut Not for Catalysis.Journal of Microbiology and Biotechnology26:1701-1707.

[3] Liu S, Wang M, Du G, Chen J.2016. Improving the active expression of transglutaminase inStreptomyces lividansby promoter engineering and codon optimization.BMC Biotechnology16.

[4] Bi J,Liu S, Du G, Chen J.2016. Bile salt tolerance ofLactococcus lactisis enhanced by expression of bile salt hydrolase thereby producing less bile acid in the cells.Biotechnology Letters38:659-665.

[5] Liu S, Wan D, Wang M, Madzak C, Du G, Chen J.2015. Overproduction of pro-transglutaminase fromStreptomyces hygroscopicusinYarrowia lipolyticaand its biochemical characterization.BMC Biotechnology15.

[6] Liu S, Dai J, Kang Z, Li J, Chen J, Du G.2015. Production of novel NaN3-resistant creatine amidinohydrolase in recombinant Escherichia coli (vol 6, pg 248, 2015).Bioengineered6:248-250.

[7] Xu Z,Liu S*, Lu X, Rao S, Kang Z, Li J, Wang M, Chen J*.2014. Thermal inactivation of a recombinant lipoxygenase fromPseudomonas aeruginosaBBE in the absence and presence of additives.Journal of the Science of Food and Agriculture94:1753-1757.

[8] Lu X,Liu S*, Feng Y, Rao S, Zhou X, Wang M, Du G*, Chen J.2014. Enhanced thermal stability ofPseudomonas aeruginosalipoxygenase through modification of two highly flexible regions.Applied Microbiology and Biotechnology98:1663-1669.

[9] Lu X, Zhang J,Liu S, Zhang D, Xu Z, Wu J, Li J, Du G, Chen J.2013. Overproduction, purification, and characterization of extracellular lipoxygenase ofPseudomonas aeruginosainEscherichia coli.Applied Microbiology and Biotechnology97:5793-5800.

[10] Lu X,Liu S, Zhang D, Zhou X, Wang M, Liu Y, Wu J, Du G, Chen J.2013. Enhanced thermal stability and specific activity ofPseudomonas aeruginosalipoxygenase by fusing with self-assembling amphipathic peptides.Applied Microbiology and Biotechnology97:9419-9427.

[11] Chen K, Zhang D,Liu S, Wang NS, Wang M, Du G, Chen J.2013. Improvement of transglutaminase production by extending differentiation phase ofStreptomyces hygroscopicus: mechanism and application.Applied Microbiology and Biotechnology97:7711-7719.

[12] Chen K,Liu S*, Zhang D, Wang M, Shi Z, Du G, Chen J*.2013. Altered secretary efficiency ofStreptomyces hygroscopicustransglutaminase inEscherichia coliby the pro-peptide modification.Process Biochemistry48:782-787.

[13] Chen K,Liu S, Wang G, Zhang D, Du G, Chen J, Shi Z.2013. Enhancement ofStreptomycestransglutaminase activity and pro-peptide cleavage efficiency by introducing linker peptide in the C-terminus of the pro-peptide.Journal of Industrial Microbiology & Biotechnology40:317-325.

[14] Yang Y, Zhang D,Liu S, Jia D, Du G, Chen J.2012. Expression and fermentation optimization of oxidized polyvinyl alcohol hydrolase inE. coli.Journal of Industrial Microbiology & Biotechnology39:99-104.

[15] Chen K,Liu S, Ma J, Zhang D, Shi Z, Du G, Chen J.2012. Deletion combined with saturation mutagenesis of N-terminal residues in transglutaminase fromStreptomyces hygroscopicusresults in enhanced activity and thermostability.Process Biochemistry47:2329-2334.

[16] Liu S, Zhang D, Wang M, Cui W, Chen K, Liu Y, Du G, Chen J, Zhou Z.2011. The pro-region ofStreptomyces hygroscopicustransglutaminase affects its secretion byEscherichia coli.FEMS MicrobiologyLetters324:98-105.

[17] Liu S, Zhang D, Wang M, Cui W, Chen K, Du G, Chen J, Zhou Z.2011. The order of expression is a key factor in the production of active transglutaminase inEscherichia coliby co-expression with its pro-peptide.Microbial Cell Factories10.

[18] Liu S, Zhang D, Liu L, Wang M, Du G, Chen J.2010. Enhanced water absorption of wheat gluten by hydrothermal treatment followed by microbial transglutaminase reaction.Journal of the Science of Food and Agriculture90:658-663.

发明专利

授权发明专利3项,申请国家(国际)发明专利30项。部分代表性专利如下:

[1] Song Liu, Yue Feng, Jian Chen, Guocheng DU, Yun Jiao, Jie Ruan, Hongye Cheng, Hui Gao. Mutant with enhanced secretion of L-asparaginase and its application. United States Patent 9896678B2

[2] Jian Chen, Guocheng Du, Xinyao Lu,Song Liu, Juan Zhang. Method for increasing protein thermal stability. United States Patent 9040274

[3] 刘松冯岳陈坚堵国成陈双全王广圣陈璇一种酶活提高的天冬酰胺酶突变体专利号:201410568323.0

获奖及荣誉:

获奖

[1] 张娟,陈坚,堵国成,刘松,康振,李江华.微生物发酵法生产新型饲料酶关键技术. 2017年中国商业联合会科学技术奖一等奖

[2] 罗玮,刘松,张薄博,杨艳坤.微生物菌种制备原理与技术. 2013年教育部第十三届全国多媒体课件大赛优秀奖

技术支持:信息化建设与管理中心

地址:江苏省无锡市蠡湖大道1800号

邮编:214122

联系电话:0510-85328539

服务邮箱:nelcft@jiangnan.edu.cn